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Table 2 Effect of inhibitors on the hydrolysis of Abz-GFSPFRQ-EDDnp by B16F10-Nex2 supernatant and recombinant TOP and Neurolysin

From: Characterization of thimet oligopeptidase and neurolysin activities in B16F10-Nex2 tumor cells and their involvement in angiogenesis and tumor growth

Inhibitors

Relative hydrolysis (%)1

B16F10-Nex2

rTOP

rNeurolysin

Control (no inhibitor)

100

100

100

o-phenanthrolin (4 mM)

0

0

0

JA-2 (3 μM)

2.6 ± 2.1

0.6 ± 1.2

1 ± 1

Pro-Ile (1 mM)

60 ± 6

87 ± 5

52 ± 4

PMSF (0.1 mM)

94 ± 6

91 ± 4

93 ± 4

E64 (0.1 mM)

88 ± 3

98 ± 2

99 ± 2

Leupeptin (0.2 mM)

81 ± 6

96 ± 2

96 ± 4

Z-Pro-Prolinal (1 μM)

93 ± 1

88 ± 3

92 ± 4

Captopril (20 μM)

92 ± 3

97 ± 2

98 ± 2

  1. 1100% hydrolysis represents the cleavage of 20 μM of Abz-GFSPFRQ-EDDnp by B16F10-Nex2 supernatant and recombinant enzymes during 5 min at 37°C in 50 mM Tris-HCl, pH 7.4. The values are means of three independent experiments.
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Molecular Cancer

ISSN: 1476-4598