AICAR treatment on ALL cells results in activation of Akt. Western blot analysis depicting the level of phosphorylated Akt protein (P-Akt, Ser473) in CCRF-CEM, NALM6, REH, and SupB15 cells were treated for 24 h with various concentrations of AICAR (0 – 0.5 mM). β-actin was used as control for the amount of proteins loaded per lane. Density value of each band was normalized to their respective β-actin level and expressed relative to control (untreated). The immunoblots shown are representative of at least 3 independent experiments.