Figure 2

A) Array-CGH ideograms of the most frequently observed chromosomal anomalies in trisomy 19 ependymomas. Blue dots: ratio between tumor DNA and control DNA; red dots: ratio between control DNA and tumor DNA. Ratio of 1 indicates normal DNA content (presence of two alleles, chromosome 2: A6). Separation of the lines corresponds to gain of tumoral DNA (blue dots going up, chromosome 19: A1, A2 and A9 and, chromosome 11: A4) or loss of tumoral DNA (blue dots going down, chromosome 13: A6 and, chromosome 9: A4). B) Scheme of array-CGH results of trisomy 19 ependymomas. Altogether 118 genetic anomalies detected, mean: 13 per tumor, consisting of 74 gains (64%) and 44 losses (36%). Numbers correspond to tumors. C) Genetic alterations presented at least in 66% (6/9) of trisomy 19 ependymomas (A1-A9) compared to two controls (Ep.: B1 and B19). Trisomy 19 observed in all cases, although in one tumor (A4) the telomeric long arm was not amplified. Deletion of 13q21.31-31.2 and deletions on chromosme 9 (M: monosomy; M: monosomy without 9qter loss; 9p: 9p deletion and Int: intertitial p and q deletions) were found in 7/9 tumors (78%). Amplification of 11q13.3-13.4 was detected in 6/9 (66%) of the tumors.