Analyzed promoter region of the RASSF1A gene. CpG sites in the promoter region of the RASSF1A gene analyzed by combined bisulfite restriction analysis (COBRA) and bisulfite sequence analysis. Numbers refer to the position of transcription start site while bracketed numbers indicate CpG sites. Solid triangles show TaqI restrictions sites used for COBRA. The solid and dashed lines indicate sequences amplified for COBRA and sequence analysis following bisulfite conversion of DNA. Inner primer positions were indicated by solid arrows.