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Figure 5 | Molecular Cancer

Figure 5

From: RASSF1A promoter methylation and expression analysis in normal and neoplastic kidney indicates a role in early tumorigenesis

Figure 5

Bisulfite sequencing of pooled tumoral and normal RASSF1A promoters. RASSF1A promoter amplicons from 42 tumoral and 42 normal tissue samples were normalized, pooled, subcloned and each 40 subclones of the tumor (T) and normal (P) group analyzed by bisulfite sequencing. For each clone the methylation status of analyzed CpG sites is shown (solid circles: methylation, open circles: no methylation). Numbers on x-axis refer to base pair positions as indicated in Figure 1. Asterisks indicate amplicons derived from densely methylated promoters demonstrating at least 11 methylated CpG sites.

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Molecular Cancer

ISSN: 1476-4598