Figure 1

An overview of the SAGE process. The SAGE method for the comprehensive analysis of gene expression patterns consists of the following steps; 1. SAGE tags containing sufficient information to uniquely identify a transcript are isolated by amplification; 2. Tags are then linked and sequenced; 3. The resulting sequence data are analyzed to identify each gene expressed in the sample and the levels at which each gene is expressed; 4. This information forms a library that can be used to compare gene expression between tissues or cell types. For a review see [14].