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Table 2 Antibodies used for immunobloting and immunohistochemistry.

From: Phosphorylation of Ser78 of Hsp27 correlated with HER-2/neu status and lymph node positivity in breast cancer

Antibodies

Stock conc. (mg/ml)

Dilution

Cat. no.

Sources

Hsp27 (Mouse monoclonal)

0.2

1:1000

Sc-13132

Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA

p Ser78 (Mouse monoclonal)

1

1:1000

05–645

Upstate Biotechnology Inc., Lake Placid, NY, USA

p-Ser82 (Rabbit polyclonal)

1

1:1000

07–489

Upstate Biotechnology Inc.

p-Ser15 (Rabbit polyclonal)

1

1:1000

07–388

Upstate Biotechnology Inc.