Figure 3

Confirmation of microarray data. (A) Northern blot analysis of miRNAs miR-25 and miR-92. As expected from microarray data, Northern blot analysis confirmed that these miRNAs were up-regulated in MSS samples. miRNA/U6 ratio of densitometric intensities is shown below each miRNA lane. (B) Quantitative Real time RT-PCR validation of microarray results. Microarray expression values of a selected panel of 5 genes differentially expressed between MSS and MSI-H groups of colorectal cancers were validated with quantitative real-time PCR. Values for microarray data were normalized on chip 50th percentile while for real-time PCR values are equal to ΔCt*10^6.