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Figure 1 | Molecular Cancer

Figure 1

From: Selective repression of retinoic acid target genes by RIP140 during induced tumor cell differentiation of pluripotent human embryonal carcinoma cells

Figure 1

RIP140 silencing enhances ligand-dependent RAR activation and tumor cell differentiation. (A, left) Northern analysis depicting the effect of RIP140 siRNA on expression of known retinoic acid receptor target gene RARβ in NT2/D1 cells. Cells were mock transfected (no siRNA) or transfected with 150 nM siRNA prior to treatment with 1 μM RA for 24 hours. (A, right) Northern analysis depicting the effect of RIP140 siRNA on expression of RARβ. Cells were transfected with 40 nM siRNA prior to treatment with 1 μM RA for 24 hours. Scbl, scrambled. B) Immunophenotypic FACS analysis for the established neuroectodermal marker A2B5 in NT2/D1 cells transfected with 40 nM scrambled siRNA (Scbl) or RIP140 siRNA#1 or RIP140 siRNA#2 and treated for 4 days with DMSO vehicle control or indicated dosages of RA. Representative of two experiments. (C) The effect of RIP140 siRNAs and exogenous RIP140 expression on activity of an RARE-TK-Luc promoter in NT2/D1 cells. Cells were transfected with indicated control (scbl) or RIP140 siRNAs at a concentration of 50 nM and either empty expression vector or a plasmid expressing RIP140 prior to addition of indicated dosages of RA for 48 hours. Note, RIP140 siRNA#1 targets sequences within the RIP140 expression plasmid while RIP140 siRNA#3 targets the 3' untranslated region of RIP140 but does not target PSG5-RIP140. Error bars are S.D. of triplicate determinations. For all experiments above, cells were cultured in charcoal absorbed sera media for 24 hours prior to and during the experiment.

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