Figure 2
From: Differential effects of hypoxia on etoposide-induced apoptosis according to the cancer cell lines
Effect of hypoxia on the etoposide-induced apoptosis. A549, MCF-7 or HepG2 cells were incubated under normoxic (N) or hypoxic (H) conditions with or without etoposide (e, 50 μM) for 40 hours. A, PARP-1 and cleaved 85 kDa fragment were detected in total cell extracts by western blotting, using a specific anti-PARP-1 antibody. a-tubulin was used to assess the total amount of proteins loaded on the gel. B, LDH release was assessed. Results are presented in percentages, as means ± 1 SD (n = 3).