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Figure 5 | Molecular Cancer

Figure 5

From: Responses of cancer cells with wild-type or tyrosine kinase domain-mutated epidermal growth factor receptor (EGFR) to EGFR-targeted therapy are linked to downregulation of hypoxia-inducible factor-1α

Figure 5

Downregulation of HIF-1α protein levels in cell lines with wild-type or mutated EGFR after treatment with cetuximab or gefitinib. Cells from the indicated cell lines were treated with cetuximab or gefitinib overnight as described in Figure 4. After treatment, the cells were lysed, and equal amounts of cell lysates were subjected to Western blot analysis using antibodies directed against HIF-1α, as indicated. The level of β-actin served as the internal control for equal protein loading in each lane. The numeric values shown under each gel were derived from a densitometric analysis of the signals.

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Molecular Cancer

ISSN: 1476-4598