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Table 1 Cell cycle analysis of wild type and PP2Cα siRNA-expressing MCF7 cells.

From: Role of PP2Cα in cell growth, in radio- and chemosensitivity, and in tumorigenicity

Time (hours post trypsination) MCF7-wt MCF7-si
  G0/G1 S G2/M G0/G1 S G2/M
Control 54.5 ± 1.5 33.5 ± 0.4 12.0 ± 1.3 44.9 ± 2.4 * 32.4 ± 1.6 22.7 ± 3.7 *
6 74.0 ± 0.5 17.3 ± 0.2 8.6 ± 0.4 50.2 ± 2.8 * 34.4 ± 2.4 * 16.1 ± 4.2 *
12 74.1 ± 0.2 16.7 ± 0.8 9.2 ± 0.7 46.1 ± 3.6 * 33.5 ± 3.1 * 20.4 ± 6.7 *
24 48.0 ± 0.9 41.7 ± 0.4 10.3 ± 0.9 57.8 ± 2.7 * 23.8 ± 3.5 * 18.4 ± 1.1 *
48 48.4 ± 0.7 39.9 ± 0.4 11.6 ± 0.8 59.4 ± 4.8 * 25.1 ± 0.3 * 15.5 ± 4.9
72 51.9 ± 0.5 34.6 ± 0.5 13.5 ± 0.3 46.3 ± 2.7 * 31.3 ± 1.0 * 21.8 ± 2.5 *
  1. Multicycle algorithm-based quantification of the amounts of MCF7-wt and MCF7-si cells in the G0/G1, the S and the G2/M phase of the cell cycle. Results are shown both for confluently growing control cells and for cells analyzed at various time points after trypsination and (re-) seeding. Values represent average ± SD (n = 3). * Indicates p < 0.05 vs. MCF-wt.