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Table 3 Cell cycle analysis of wild type and PP2Cα siRNA-expressing MCF7 cells upon treatment with chemotherapy.

From: Role of PP2Cα in cell growth, in radio- and chemosensitivity, and in tumorigenicity

   MCF7-wt MCF7-si
Time (h) Dox Dose (μM) G0/G1 S G2/M G0/G1 S G2/M
24 0 53.6 ± 0.5 33.3 ± 1.2 13.1 ± 0.3 56.6 ± 0.5 * 29.7 ± 0.6 * 13.7 ± 0.9
  1 74.8 ± 0.9 8.0 ± 0.6 17.2 ± 0.3 53.9 ± 0.6 * 30.7 ± 1.0 * 15.4 ± 1.3
  2.5 50.4 ± 0.7 4.1 ± 0.8 45.5 ± 1.5 52.1 ± 0.9 5.0 ± 2.7 42.8 ± 1.9
  5 45.1 ± 0.2 6.4 ± 0.2 48.5 ± 0.1 51.5 ± 1.6 * 5.1 ± 2.4 43.4 ± 0.9 *
  10 38.9 ± 1.3 29.5 ± 0.8 31.6 ± 0.8 41.3 ± 0.3 * 32.5 ± 1.6 * 26.2 ± 1.5 *
  20 40.8 ± 1.8 31.3 ± 2.5 27.8 ± 1.3 54.9 ± 1.4 * 30.2 ± 0.5 14.9 ± 1.4 *
48 0 61.5 ± 0.6 29.1 ± 1.0 9.4 ± 0.7 61.2 ± 1.8 29.5 ± 1.7 11.2 ± 0.1 *
  1 71.4 ± 3.5 8.9 ± 1.7 19.7 ± 5.2 52.2 ± 2.7 * 2.5 ± 1.2 * 45.3 ± 1.7 *
  2.5 35.7 ± 1.3 3.9 ± 0.3 60.4 ± 1.0 58.3 ± 5.8 * 3.2 ± 0.6 38.5 ± 5.3 *
  5 31.7 ± 0.5 5.2 ± 1.6 63.2 ± 1.1 49.6 ± 1.0 * 2.5 ± 0.7 47.8 ± 0.4 *
  10 30.3 ± 1.0 23.6 ± 0.8 46.1 ± 0.7 44.0 ± 1.7 * 8.4 ± 0.6 * 47.6 ± 2.3
  20 33.2 ± 1.8 48.6 ± 7.7 18.3± 9.4 50.3 ± 0.8 * 43.4 ± 2.4 6.4 ± 1.6
72 0 71.9 ± 1.7 22.8 ± 0.7 5.4 ± 1.3 59.2 ± 0.9 * 30.4 ± 1.6 * 10.5 ± 0.6 *
  1 52.5 ± 7.2 3.1 ± 1.9 44.4 ± 8.9 47.4 ± 7.3 0.5 ± 0.9 52.1 ± 8.2
  2.5 46.4 ± 1.2 0.5 ± 0.5 53.3 ± 2.0 41.3 ± 2.6 * 1.9 ± 3.0 56.8 ± 1.8
  5 38.8 ± 0.3 0.1 ± 0.1 61.3 ± 0.3 40.4 ± 0.8 * 1.8 ± 1.6 57.8 ± 1.5 *
  10 34.1 ± 0.7 16.4 ± 1.7 49.5 ± 2.3 37.5 ± 1.5 * 3.5 ± 2.5 * 57.1 ± 2.6 *
  20 32.8 ± 2.8 39.9 ± 1.8 27.3 ± 1.8 N.Q. N.Q. N.Q.
  1. Multicycle algorithm-based quantification of the amounts of MCF7-wt and MCF7-si cells in the G0/G1, the S and the G2/M phase of the cell cycle at 24, 48 and 72 h after treatment with the indicated doses of doxorubicin. Values represent average ± SD (n = 3). N.Q. (Not Quantifiable) indicates that multicycle algorithm failed to attribute the cells to a certain cell cycle phase. * Indicates p < 0.05 vs. MCF-wt.