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Table 3 Cell cycle analysis of wild type and PP2Cα siRNA-expressing MCF7 cells upon treatment with chemotherapy.

From: Role of PP2Cα in cell growth, in radio- and chemosensitivity, and in tumorigenicity

  

MCF7-wt

MCF7-si

Time (h)

Dox Dose (μM)

G0/G1

S

G2/M

G0/G1

S

G2/M

24

0

53.6 ± 0.5

33.3 ± 1.2

13.1 ± 0.3

56.6 ± 0.5 *

29.7 ± 0.6 *

13.7 ± 0.9

 

1

74.8 ± 0.9

8.0 ± 0.6

17.2 ± 0.3

53.9 ± 0.6 *

30.7 ± 1.0 *

15.4 ± 1.3

 

2.5

50.4 ± 0.7

4.1 ± 0.8

45.5 ± 1.5

52.1 ± 0.9

5.0 ± 2.7

42.8 ± 1.9

 

5

45.1 ± 0.2

6.4 ± 0.2

48.5 ± 0.1

51.5 ± 1.6 *

5.1 ± 2.4

43.4 ± 0.9 *

 

10

38.9 ± 1.3

29.5 ± 0.8

31.6 ± 0.8

41.3 ± 0.3 *

32.5 ± 1.6 *

26.2 ± 1.5 *

 

20

40.8 ± 1.8

31.3 ± 2.5

27.8 ± 1.3

54.9 ± 1.4 *

30.2 ± 0.5

14.9 ± 1.4 *

48

0

61.5 ± 0.6

29.1 ± 1.0

9.4 ± 0.7

61.2 ± 1.8

29.5 ± 1.7

11.2 ± 0.1 *

 

1

71.4 ± 3.5

8.9 ± 1.7

19.7 ± 5.2

52.2 ± 2.7 *

2.5 ± 1.2 *

45.3 ± 1.7 *

 

2.5

35.7 ± 1.3

3.9 ± 0.3

60.4 ± 1.0

58.3 ± 5.8 *

3.2 ± 0.6

38.5 ± 5.3 *

 

5

31.7 ± 0.5

5.2 ± 1.6

63.2 ± 1.1

49.6 ± 1.0 *

2.5 ± 0.7

47.8 ± 0.4 *

 

10

30.3 ± 1.0

23.6 ± 0.8

46.1 ± 0.7

44.0 ± 1.7 *

8.4 ± 0.6 *

47.6 ± 2.3

 

20

33.2 ± 1.8

48.6 ± 7.7

18.3± 9.4

50.3 ± 0.8 *

43.4 ± 2.4

6.4 ± 1.6

72

0

71.9 ± 1.7

22.8 ± 0.7

5.4 ± 1.3

59.2 ± 0.9 *

30.4 ± 1.6 *

10.5 ± 0.6 *

 

1

52.5 ± 7.2

3.1 ± 1.9

44.4 ± 8.9

47.4 ± 7.3

0.5 ± 0.9

52.1 ± 8.2

 

2.5

46.4 ± 1.2

0.5 ± 0.5

53.3 ± 2.0

41.3 ± 2.6 *

1.9 ± 3.0

56.8 ± 1.8

 

5

38.8 ± 0.3

0.1 ± 0.1

61.3 ± 0.3

40.4 ± 0.8 *

1.8 ± 1.6

57.8 ± 1.5 *

 

10

34.1 ± 0.7

16.4 ± 1.7

49.5 ± 2.3

37.5 ± 1.5 *

3.5 ± 2.5 *

57.1 ± 2.6 *

 

20

32.8 ± 2.8

39.9 ± 1.8

27.3 ± 1.8

N.Q.

N.Q.

N.Q.

  1. Multicycle algorithm-based quantification of the amounts of MCF7-wt and MCF7-si cells in the G0/G1, the S and the G2/M phase of the cell cycle at 24, 48 and 72 h after treatment with the indicated doses of doxorubicin. Values represent average ± SD (n = 3). N.Q. (Not Quantifiable) indicates that multicycle algorithm failed to attribute the cells to a certain cell cycle phase. * Indicates p < 0.05 vs. MCF-wt.