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Figure 1 | Molecular Cancer

Figure 1

From: A novel activating role of SRC and STAT3 on HGF transcription in human breast cancer cells

Figure 1

Src/Stat3 mediated HGF promoter transactivation in mouse epithelial breast cell lines. A DNA sequence alignment between the mouse HGF proximal promoter spanning from nt-274 to +29 is shown with the comparable region of the human gene (A). The Stat3 site at nt-95 is boxed and the transcriptional start site is indicated by an arrow. The 0.3 HGF-Renilla or -95 M 0.3 HGF-Renilla mouse proximal promoters were co-transfected with either activated Src or Stat3 alone, or in combination in: mouse mammary carcinoma SP1 cells (B), non-tumorigenic mouse mammary HC11 cells (C), and non-tumorigenic mouse mammary EPH4 cells (D). 48 hours post-transfection, cells were lysed and assayed for dual-luciferase activity. Values were normalized using a CMV-Luc internal control and are presented as fold induction relative to the empty vector (EV) for 0.3 HGF-Renilla. Asterisks indicate a significant increase in HGF promoter activity compared to the 0.3 HGF-Renilla empty vector control (P = 0.001*, P = 0.0005** using a Student's T-test). Transfections were repeated three times and values represent average results of triplicate samples +/- SD.

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