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Figure 2 | Molecular Cancer

Figure 2

From: A novel activating role of SRC and STAT3 on HGF transcription in human breast cancer cells

Figure 2

Endogenous expression and activity of HGF/Met signaling proteins in mouse and human epithelial cell lines. Endogenous protein expression and activation levels of HGF, Met, Src and Stat3 were characterized in mouse and human epithelial cell lines by western blot analysis. Whole cell lysates collected from the indicated cell lines were normalized and resolved on a reducing SDS-PAGE gel. Western blot analysis was performed using antibodies probing for the indicated proteins (arrows point to relevant band). γ-Tubulin was used as a loading control to normalize for overall protein concentration, and recombinant HGF (rHGF) served as a control for HGFα migration.

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