Figure 3

Src/Stat3 mediated HGF promoter transactivation in human epithelial breast cell lines. The 0.3 HGF-Renilla or -95 M 0.3 HGF-Renilla mouse proximal promoters were co-transfected with either activated Src or Stat3 alone, or in combination in: human non-tumorigenic 184-hTERT (A) and MCF10a breast cells (B), human MCF-7 adenocarcinoma breast cells (C), human T47-D ductal carcinoma breast cells (D), and human SK-BR-3 adenocarinoma breast cells (E). 48 hours post-transfection, cells were lysed and assayed for dual-luciferase activity. Values were normalized using a CMV-Luc internal control and are presented as fold induction relative to the empty vector (EV) for 0.3 HGF-Renilla. Asterisks indicate a significant increase in HGF promoter activity compared to the 0.3 HGF-Renilla empty vector control (P = 0.001*, 0.0003**, 0.0005*** using a Student's T-test). Transfections were repeated three times and values represent average results of triplicate samples +/- SD.