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Figure 4 | Molecular Cancer

Figure 4

From: A novel activating role of SRC and STAT3 on HGF transcription in human breast cancer cells

Figure 4

Src/Stat3 mediated HGF promoter transactivation in human non-breast epithelial cell lines. The 0.3 HGF-Renilla or -95 M 0.3 HGF-Renilla mouse proximal promoters were co-transfected with either activated Src or Stat3 alone, or in combination in: human ovarian tumorigenic OVCAR3 (A) and SKOV3 cells (B), human ovarian non-tumorigenic ovarian IOSE-80PC cells (C), human embryonic kidney HEK293 adenocarcinoma cells (D), and human cervical carcinoma HeLa cells (E). 48 hours post-transfection, cells were lysed and assayed for dual-luciferase activity. Values were normalized using a CMV-Luc internal control and are presented as fold induction relative to the empty vector (EV) for 0.3 HGF-Renilla. Asterisks indicate a significant increase in HGF promoter activity compared to the 0.3 HGF-Renilla empty vector control (P = 0.0005* using a Student's T-test). Transfections were repeated three times and values represent average results of triplicate samples +/- SD.

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