Transcriptional changes associated with breast cancer occur as normal human mammary epithelial cells overcome senescence barriers and become immortalized

Table 1 Cell Types and Lines Used in This Study

Cell Name	Source	Stage	Growth Media
48L	LBNL	Pre-stasis, finite lifespan strain	MM (MEBM)***
161	LBNL	Pre-stasis, finite lifespan strain	MM (MEBM)
184	LBNL	Pre-stasis, finite lifespan strain	MM (MEBM)
195L	LBNL	Pre-stasis, finite lifespan strain	MM (MEBM)
48R	LBNL	Post-selection, finite lifespan strain	MEBM
161	LBNL	Post-selection, finite lifespan strain	MEBM
184	LBNL	Post-selection, finite lifespan strain	MEBM
195L	LBNL	Post-selection, finite lifespan strain	MEBM
191	LBNL	Post-selection, finite lifespan strain	MEBM
239	LBNL	Post-selection, finite lifespan strain	MEBM
HMEC-1001-13	Clonetics	Post-selection, finite lifespan strain**	MEBM
184A1	LBNL	Fully immortal cell line	MEBM
184B5	LBNL	Fully immortal cell line	MEBM
184AA2	LBNL	Fully immortal cell line	MEBM
184AA3	LBNL	Fully immortal cell line	MEBM
184B5ME	LBNL	Fully immortal cell line	MEBM
184A1*	ATCC	Fully immortal cell line	DMEM/F-12
184B5*	ATCC	Fully immortal cell line	DMEM/F-12
MCF-10A	ATCC	Fully immortal cell line	DMEM/F-12
MCF-10A-2	ATCC	Fully immortal cell line	DMEM/F-12
MCF-12A	ATCC	Fully immortal cell line	DMEM/F-12

*designated as 184A1(a) and 184B5(a) in other tables and figures
**stage defined by transcriptional profile
***cells isolated from reduction mammoplasty tissues and expanded in MM media to passages 2–3, then cultured in serum-free MEBM media for transcriptional profiling

ISSN: 1476-4598