Table 1 Primer sequences used for real-time PCR
Target mRNA | Forward primer (5'→ 3') | Reverse primer (5'→ 3') | Product size (bp) |
|---|---|---|---|
β-actin | TGACAGGATGCAGAAGGAGA | CGCTCAGGAGGAGCAATG | 75 |
ALP | AAGGCTTCTTCTTGCTGGTG | GCCTTACCCTCATGATGTCC | 61 |
ATF4 | ATGATGGCTTGGCCAGTG | TCTCCAACATCCAATCTGTCC | 72 |
Cbfa1 | GATGACACTGCCACCTCTGA | GCCCAGTTCTGAAGCACCT | 81 |
M-CSF | CAGCTGCTTCACCAAGGACT | TCATGGAAAGTTCGGACACA | 60 |
OCN | AGACTCCGGCGCTACCTT | CTCGTCACAAGCAGGGTTAAG | 93 |
OPG | ATGAACAAGTGGCTGTGCTG | CAGTTTCTGGGTCATAATGCAA | 106 |
Osterix | TTTAAACAAACACGATGATGATGA | ATTGGACTTCCCCCTTCTTG | 138 |
RANKL | TGAAGACACACTACCTGACTCCTG | CCACAATGTGTTGCAGTTCC | 83 |