Figure 3
Proteasome activity and expression of catalytic subunits upon bortezomib treatment. (A) Intracellular proteasome activity in H460, H460-Bcl-2 and SW1573 cells treated with a concentration range of bortezomib. Mean of at least three independent experiments, SD. (B) Time and concentration course Western blot analysis of total cell extracts of H460 and SW1573 cells showing the effect with treatment with bortezomib on the expression level of the 20S proteasome core (α/β), individual catalytic subunits: β1,β2, β5 and (C) heat shock proteins Hsp27, Hsp70 and Hsp90.