Figure 3From: The oncoprotein H-RasV12 increases mitochondrial metabolismNMR-based isotopomer analysis of intracellular glutamate/glutamine synthesis from glucose. The regions of 2D TOCSY spectra obtained from NHBE, hT/LT and hT/LT/Ras cells specific for the combined glutamate/glutamine, were analyzed for relative 13C enrichment. Spectra from NHBE, hT/LT and hT/LT/Ras cells are shown. 13C isotopomers were quantified by indirect detection of protons in the 2D TOCSY NMR spectrum and % 13C-enrichment is indicated adjacent to the cross peaks.Back to article page