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Figure 5 | Molecular Cancer

Figure 5

From: Antiproliferative and pro-apoptotic activity of eugenol-related biphenyls on malignant melanoma cells

Figure 5

Effect of S7-( S ) on cell viability and apoptosis in melanoma cells. (A) Melanoma cells and normal fibroblasts were incubated with 50 μM S7-(S) or solvent (control) for 24 hours, and cell death was determined by trypan blue staining. Results are expressed as mean number of trypan blue-positive cells in triplicate cultures from two independent experiments. Cells were also pre-treated for 1 hour with the pan-caspase inhibitor before S7-(S) treatment. P values for S7-(S) versus control and for inhibitor versus S7-(S) were calculated by Student's t-test with Welch correction: *, P < .05; **, P < .01. (B) Effect of S7-(S) on phosphatidylserine exposure on melanoma cells. Human fibroblasts and melanoma cell lines were incubated in the presence or absence of 50 μM S7-(S) for 24 hours. Cells were also pre-treated for 1 hour with the pan-caspase inhibitor before S7-(S) treatment. Cells were double stained with annexin V-FITC to detect phosphatidylserine exposure and propidium iodide to detect DNA and analysed by flow cytometry. Results are expressed as mean percentage of apoptotic cells from three independent experiments. P values for S7-(S) versus control and for inhibitor versus S7-(S) were calculated by Student's t-test with Welch correction: *, P < .05; **, P < .01; ***, P < .001. (C) Effect of S7-(S) on DNA fragmentation in melanoma cells. GR-mel (a, d), Sbcl2 (b, e) melanoma cell lines and human fibroblasts (c, f) were treated with 50 μM (d, e, f) S7-(S) or grown in S7-(S) free medium (control a, b, c) for 24 hours and apoptotic cells (red) were stained by the TUNEL assay. Cell nuclei (blue) were stained with DAPI.

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