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Figure 5 | Molecular Cancer

Figure 5

From: Human periostin gene expression in normal tissues, tumors and melanoma: evidences for periostin production by both stromal and melanoma cells

Figure 5

Comparison of POSTN expression in melanoma tumors and cell lines. A. A total of 23 melanoma cell lines (black bars) and matched metastatic tumors (grey bars) were assessed for POSTN and ACTB expression in triplicate as described in Fig. 1 legend. All melanoma cell lines were established in our laboratory and subjected to no more than 4 to 11 passages before RNA extraction. Metastases were isolated from tumor-invaded lymph nodes (LB2201-2, LB2730-1, LB2165-1, LB2117-3, LB2667-1a, LB2800, LB1448-2, LB2870, EB81-2), skin (DDHK0062, KUL81-2), sub-cutaneous tissues (EB124, CP50-1b, LB2174-1, LB2174-3, LB2077-1, LB2821, LB2181-2, LB2763-1, LB2259-1, LB2307-2, VUB105) or heart (LB2077-4). Error bars represent SD. B. POSTN expression level was measured in independent melanoma cell lines derived from distinct metastasis of patients LB2174, LB2730 and LB2077. Date of cell line establishment (MM.YY) is indicated above the bars. C. POSTN and COL6A3 cDNA levels were measured by qRT-PCR in 12 melanoma cell lines and matched tumors. Values are given as (expression in cell line/expression in tumor) × 100%. D. Periostin protein was analyzed in conditioned medium from LB2077-1 and LB2730-1 periostin-producing melanoma cell lines and from LB2181-2 and LB2667-1a melanoma cell lines, which do not express POSTN, as negative controls.

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Molecular Cancer

ISSN: 1476-4598