The effect of pancreatic cancer cell-conditioned media (CM) on 3H-thymidine incorporation by mink lung epithelial (CCL-64) cells. CCL-64 cells were exposed to non-activated CM (with only active TGFβ, top panels) and acid-activated CM (total TGFβ, bottom panels) from CAPAN-2 and Hs766T cells treated with retinoic acid for 48 hours. The growth of the CCL-64 cells was significantly attenuated by both types of CM. One way ANOVA: Capan-2, non-activated CM: F(3,11) = 40.6, P < 0.0001; Capan-2, activated CM: F(3,11) = 16.5, P = 0.0009; Hs766T, non-activated CM: F(2,11) = 39.2, P < 0.0001; Hs766T, activated CM: F(2,11) = 231, P < 0.0001. Data are expressed as counts per minute (CPM), bars represent means ± SEM. Significance of individual comparisons after Dunnett's multiple comparison test is indicated by ** = P < 0.01.