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Figure 5 | Molecular Cancer

Figure 5

From: BGLAP is expressed in pancreatic cancer cells and increases their growth and invasion

Figure 5

Expression and localization of BGLAP in four cultured pancreatic cancer cell lines. (A) QRT-PCR analysis of mRNA levels for BGLAP in pancreatic cancer cells was carried out as described in the Methods section. RNA input was normalized to the average expression of the two housekeeping genes HPRT and cyclophilin B. Bars represent the mean values +/- SEM of 3 independent experiments as adjusted transcripts/10,000 CPB copies. (B) Enzyme immunoassay of BGLAP levels in cell culture supernatant of pancreatic cancer cell lines was carried out as described in the Methods section. Bars represent the mean values +/- SEM of 3 independent experiments in ng/ml. Localization of BGLAP in the cultured pancreatic cancer cell lines was performed by immunocytochemistry as described in the Methods section, using a specific BGLAP antibody (+). The specificity of the staining was confirmed using control IgG (-).

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