Table 6 List of primer. Primer sets for qRT-PCR and sqRT-PCR
Gene name | Accession No. | Quantitative or sqRT-PCR primer sequence |
|---|---|---|
CXCR4 | Â | Â |
Upstream | D87747 | CATGGAACCGATCAGTGTGA (325)* |
Downstream | Â | TTTCCCAAAGTACCAGTCAGC |
MMP2 | Â | Â |
Upstream | NM_008610 | CTGTGTTCTTCGCAGGGAAT (433) |
Downstream | Â | TGTGCAGCGATGAAGATGAT |
Snail2 | Â | Â |
Upstream | NM_011415 | TTCCTCTGACACTTCATCCAA (474) |
Downstream | Â | TTGGAGCAGTTTTTGCACTG |
E-tcad | Â | Â |
Upstream | NM_009864 | CCTGCCAATCCTGATGAAAT (329) |
Downstream | Â | TCAGGGA AGGAGCTGAAAGA |
Fgf13 | Â | Â |
Upstream | AF020737 | CATTTTCTGCCCAAACCACT (378) |
Downstream | Â | AATGCTTGGCACTCTTTTGC |
Rsk4 | Â | Â |
Upstream | BB402211 | GTGGGTGCCAAAGTTTTGAT (351) |
Downstream | Â | CAAACCACATGGAAATCAGG |
MIF | Â | Â |
Upstream | NM_010798.1 | ACTACAGTAAGCTGCTGTGTGG (208) |
Downstream | Â | ATCGCTACCGGTGGATAAAC |
Mcm7 | Â | Â |
Upstream | NM_008568.1 | ACCGCGAAGTCAGTACACAA (208) |
Downstream | Â | GATGGTCTGCTGCTCCATAA |
Ttr | Â | Â |
Upstream | NM_013697.1 | TGGAAGACACTTGGCATTTC (194) |
Downstream | Â | TGCTACTGCTTTGGCAAGAT |
H2Aa | Â | Â |
Upstream | NM_010378.2 | CCTTCATCCCTTCTGACGAT (197) |
Downstream | Â | CAGGCCTTGAATGATGAAGA |
Mrpl19 | Â | Â |
Upstream | NM_026490.2 | TGCATCCCATGAAGAAGAGA (183) |
Downstream | Â | GACATTTGCTCGTTACAAAAGC |
Dusp9 | Â | Â |
Upstream | NM_029352.3 | CCTGTGCTTGAGCTCTGATT (181) |
Downstream | Â | GCTCTCCAAATTGGCTGAAT |
S100g | Â | Â |
Upstream | NM_009789.2 | CAGCAAAATGTGTGCTGAGA (197) |
Downstream | Â | CTCCATCGCCATTCTTATCC |
Serpina1a | Â | Â |
Upstream | NM_009243 | GCCCTGGCAAATTACATTCT (196) |
Downstream | Â | CATTGCCTGCATAATCCATC |
Peg3 | Â | Â |
Upstream | NM_008817.2 | ACCATTCAGGCCTCAGTTTC (205) |
Downstream | Â | TTTTCTCAAATTCGCTGACG |
Igfbp1 | Â | Â |
Upstream | NM_008341 | CCTGCCAACGAGAACTCTAT (196) |
Downstream | Â | GGGATTTTCTTTCCACTCCA |
Saa3 | Â | Â |
Upstream | NM_011315.3 | GCGAGCCTACTCTGACATGA (196) |
Downstream | Â | ATTGGCAAACTGGTCAGCTC |
Cldn18 | Â | Â |
Upstream | NM_019815.2 | GCTGTACGAGCCCTGATGAT (193) |
Downstream | Â | TGTTGGCAAACACAGACACA |
Sfrs1 | Â | Â |
Upstream | NM_173374.3 | CACTGGTGTCGTGGAGTTTG (190) |
Downstream | Â | CTTCTGCTACGGCTTCTGCT |
Sfrs2 | Â | Â |
Upstream | NM_013663.3 | GCTTTGCTTTCGTCGAATTT (188) |
Downstream | Â | AGGACTCCTCCTGCGGTAAT |
Eif2 | Â | Â |
Upstream | NM_026030.2 | GGAGTTGCTGAACCGAGTGT (180) |
Downstream | Â | AGGAGATGTTTGGGTTGACG |
Muc13 | Â | Â |
Upstream | NM_010739.1 | TGCGTGATGCTACAAAGGAC (195) |
Downstream | Â | TGTCCTGGCATTTACTGCTG |
Igfbp1 (human) | Â | Â |
Upstream | NM_000596.2 | AAGGCACAGGAGACATCAGG (195) |
Downstream | Â | TATCTGGCAGTTGGGGTCTC |
Serpina1 (human) | Â | Â |
Upstream | NM_001002235.1 | TGCCTGATGAGGGGAAACTA (186) |
Downstream | Â | CCCCATTGCTGAAGACCTTA |
WT1(362–970) |  |  |
Upstream | NC_000068 | TCCAGCAGCCGGAGCAACCT (608) |
Downstream | Â | AGGGCGTGTGGCCATAGCTG |
WT1(947–1470) |  |  |
Upstream | NC_000068 | CGCCCAGCTATGGCCACACG (523) |
Downstream | Â | ATTGCAGCCTGGGTATGCAC |
WT1(1444–1943) |  |  |
Upstream | NC_000068 | TTCATGTGTGCATACCCAGG (499) |
Downstream | Â | GTAGATCCACAGTCGTGTCC |