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Figure 6 | Molecular Cancer

Figure 6

From: Insulin-like growth factor-I gene therapy reverses morphologic changes and reduces hyperprolactinemia in experimental rat prolactinomas

Figure 6

Diagrammatic representation of the procedure used to construct RAd-IGF-I. An expression cassette containing the rat IGF-I cDNA (form B) flanked by the mCMV promoter (PmCMV) and the SV40 polyadenylation signal (SV40pA), upstream and downstream, respectively, was inserted in the shuttle vector pDC515. Subsequently, pDC515 and the genomic plasmid pBHGfrt(del)E1,3 FLP (which consists of the entire genome of adenovirus 5 (Ad 5), containing deletions in the regions E1 and E3) were cotransfected in permissive HEK 293 cells. Enzyme-directed recombination occurred in cotransfected cells, giving rise to RAd-IGFI. frt, recognition element for the yeast FLP recombinase; ITR, inverted terminal repeats; Δ1 and Δ3: deletions in the Ad 5 genome.

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Molecular Cancer

ISSN: 1476-4598