Figure 6

Cell survival after high dose oxaliplatin treatment and release. A, Number of viable cells determined after oxaliplatin treatment (1 μM, 2 μM, and 4 μM) of SW480 and SW480-PSAT1 cells. Oxaliplatin treatment lasted for three days and the first counting was done at the end of the three days treatment (D0). Cells were then washed and incubated in complete medium without oxaliplatin (release). Second counting was done three days after the oxaliplatin release (D3). Number of viable cells was determined by counting, excluding trypan blue stained cells. Data are the mean of three independent experiments. B, Cell survival analysis during the three days of oxaliplatin treatment (2 μM) followed by 11 days without drug. The number of viable cells was determined by counting, excluding trypan blue stained cells. Data are the mean of three independent experiments. C, Images of SW480-FlpIn and SW480-PSAT1 cell morphology. Day -3 corresponds to the first day of oxaliplatin treatment (2 μM). Day 0 corresponds to the last day of oxaliplatin treatment. Days 3, 7, and 11 correspond to the number of days after the oxaliplatin release. Magnification ×10.