Figure 6

Effects of curcumin and/TRAIL on markers of angiogenesis. (A), Immunohistochemistry was performed to measure the expression of VEGF in tumor tissues derived from control and treated mice on week 6. (B), Left panel, tumor tissue sections derived from control and treated mice on week 6 were stained with H & E and the number of blood vessels in ten field at 400 × magnification were counted. Each column represents the mean ± SD. * or # = significantly different from control, P < 0.05. Middle panel, blood vessel quantification in tumors derived on week 6. Tumor sections from control and treated mice were stained with anti-CD31 antibody, and the number of CD31-positive blood vessels was counted. The results are shown as the mean ± SD. * or # = significantly different from control, P < 0.05. Right panel, tumor sections from control and treated mice obtained on week 6 were stained with anti-von Willebrand Factor (vWF) antibody to evaluate blood vessels. The results are shown as the mean ± SD. (C), VEGF receptor 2 (VEGF-R2)-positive circulating endothelial cells in mice on week 6. The blood cells from peripheral blood attached to the slide were stained with anti-VEGF-R2 antibody, and the number of positive cells was counted under a microscope. The results are shown as the mean ± SD. * or # = significantly different from control, P < 0.05. (D), Expression of VEGF and β-actin in tumor tissues derived on week 6 were measured by the Western blot analysis.