Growth inhibition, IC
and apoptosis in WSU-FSCCL cells expose to ApoG2. WSU-FSCCL cells were seeded in 24 well culture plates at a density of 2 × 105 cells per 1 mL of RPMI 1640 + 10% FBS. ApoG2 was added at 0.04 μM to 10.0 μM concentrations and plates were incubated at 37°C in CO2 incubator for 24 to 72 hours. Trypan blue exclusion dye was used to determine viable cells (A). Fifty percent cell inhibition of viable cells was calculated from trypan blue exclusion assay, IC50 = 109.2 ± 18.1 nM at 72 h (B). AO/EB was quantitated by counting several fields of cells on frosted slides (C). Counting was performed using Nikon light and fluorescent microscope. AnnexinV stain was used to confirm percentage of apoptotic cells incubated for 48 and 72 h (D).