Cell viability and apoptosis of patient samples, and cell viability of peripheral blood lymphocytes exposed to ApoG2. Patient samples (A,B) and peripheral blood lymphocytes from a normal donor (C) were seeded in 24 well culture plates at a density of 4 × 105 cells per 1 mL of RPMI 1640 + 10% FBS. ApoG2 was added at 0.35 μM to 3.50 μM concentrations and plates were incubated at 37°C in CO2 incubator for 24 to 72 hours. Trypan blue exclusion dye was used to determine viable cells. Trypan blue exclusion was used to determine viable cells (A and C). Each dot represents a different patient. (-) is the mean of ApoG2 concentration that causes 50 percent growth inhibition at 72 h, Pre-B-ALL represents 24 h only (A). AO/EB was quantitated by counting fields of cells on frosted slides (B).