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Figure 4 | Molecular Cancer

Figure 4

From: Preclinical studies of Apogossypolone: a new nonpeptidic pan small-molecule inhibitor of Bcl-2, Bcl-XL and Mcl-1 proteins in Follicular Small Cleaved Cell Lymphoma model

Figure 4

Induction of caspase activation and caspase cleavage in WSU-FSCCL cells expose to ApoG2. Caspase-9 and -3 colorimetric activity assay on WSU-FSCCL cells exposed to ApoG2 at indicated times and concentrations. 50 μg of protein from cell lysates were incubated in triplicate with the corresponding substrates for caspase-9 (LEHD-pNA) caspase-3 (DEVD-pNA). Free pNA is released from the labeled synthetic substrate on cleavage by active caspase and analyzed (A and B). Protein obtained from cell lysates (100 μg) of WSU-FSCCL were separated on a 12% SDS-PAGE. Proteins were immunoblotted using specific antibodies to caspase-9, -3, and -8. WSU-FSCCL cells exposed to ApoG2 at indicated times and concentrations (0.35 μM to 3.50 μM) (C, D, and E). Primary antibodies were diluted in 2% BSA. Secondary HRP conjugates were diluted in 5% milk. Quantitation of bands: Values are fold increase of intensity over control based on percentage Integrated Density Value (IDV).

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