Induction of PARP and AIF cleavage in WSU-FSCCL cells expose to ApoG2. WSU-FSCCL cells were exposed to ApoG2 concentrations 0.35 μM to 3.50 μM for 24 to 72 h. Protein obtained from cell lysates (100 μg) of WSU-FSCCL cells were separated on a 12% SDS-PAGE. Proteins were immunoblotted using specific primary antibodies to PARP (A) and AIF (B). Primary antibodies were diluted in 2% BSA. Secondary HRP conjugates were diluted in 5% milk. Quantitation of bands: Values are fold increase of intensity over control based on percentage Integrated Density Value (IDV).