Skip to main content
Figure 3 | Molecular Cancer

Figure 3

From: Correlation between β-catenin mutations and expression of Wnt-signaling target genes in hepatocellular carcinoma

Figure 3

Effect of different mutations of β-catenin on the expression of the reporter gene 'TOP-flash'. Cells from the cell-line HuH7 were transfected with the reporter gene 'TOP-flash' harbouring LEF-1/TCF binding sites for β-catenin and the corresponding 'FOP-flash' without these sites. Each of the two reporters was cotransfected with several expression plasmids for different β-catenin forms: The construct 'bCATa' as a control not expressing any functional protein, the vector 'pBatem' expressing a truncated β-catenin, 'pCS2', expressing a form with a mutation of all N-terminal phosphorylation sites to alanine and in addition plasmids with mutations found in human HCCs: H36P, P44A, S45F and S45P. The expression from each co-transfection with 'TOP-flash' was compared to cotransfection with 'FOP-flash' in order to determine the factor of enhancement mediated by the mutants.

Back to article page