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Figure 4 | Molecular Cancer

Figure 4

From: Hypoxia induces protection against etoposide-induced apoptosis: molecular profiling of changes in gene expression and transcription factor activity

Figure 4

Cluster analysis of the gene expression patterns and transcription factor activity profiles. The expression values of up- and down-regulated genes as well as the activity profiles of the transcription factors of interest, measured with the DNA binding array and using a reporter system, were subjected to K-means clustering generating 10 clusters. A. Pseudocolors are given for increased expression/activity (red) or decreased expression/activity (green). B, Act– (highlighted in red) means the transcriptional activity measured by a reporter system for each transcription factor of interest; Db– (highlighted in yellow) is for the DNA binding activity measured with the array. C, D Cluster analysis of the gene expression patterns, the transcription factor activity profiles and the "phenotypic" profiles of the apoptosis level. C, "phenotype" values from figure 1, for caspase activity (caspactivity) and DNA fragmentation (DNAfrag) (highlighted in blue), were transformed in log values of the ratio normalized to the corresponding normoxic control. D, these values were subjected to K-means clustering generating 10 clusters. Act– means the transcriptional activity measured by a reporter system for each transcription factor of interest; Db– is for the DNA binding activity measured with the array.

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