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Figure 6 | Molecular Cancer

Figure 6

From: Hypoxia induces protection against etoposide-induced apoptosis: molecular profiling of changes in gene expression and transcription factor activity

Figure 6

A, Gene expression profiling in HepG2 cells incubated with or without etoposide under normoxic or hypoxic conditions after HIF-1α siRNA transfection. Cells were transfected with 50 nM HIF-1α siRNA or non-targeting siRNA for 24 hours. They were then incubated under normoxic (N) or hypoxic (H) conditions with or without etoposide (e, 50 μM) for 16 hours before total RNA extraction, reverse transcription and amplification by real-time PCR in the presence of SYBR Green and specific primers. α-tubulin was used as the house keeping gene for data normalization. Data are given in fold-induction as the mean ± 1 SD for experimental triplicates or as the mean for experimental duplicates. ** p < 0.01 vs hypoxia+etoposide.

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