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Table 2 Comparison of the results obtained with real time RT-PCR and DNA microarrays analyses for MCL1, BAK1, GPX1, CDNK1A and JUN genes.

From: Hypoxia induces protection against etoposide-induced apoptosis: molecular profiling of changes in gene expression and transcription factor activity

  

N

Ne

H

He

MCL1

real time RT-PCR

1

2.97

5.53

8.24

 

DNA microarray

1

3.44

3.92

5.27

BAK 1

real time RT-PCR

1

3.37

0.96

2.92

 

DNA microarray

1

3.42

0.75

2.13

GPX1

real time RT-PCR

1

4.04

0.94

2.21

 

DNA microarray

1

2.88

0.69

1.43

CDKN1A

real time RT-PCR

1

3.65

0.72

2.44

 

DNA microarray

1

3.02

0.52

1.64

JUN

real time RT-PCR

1

1.45

5.54

4.36

 

DNA microarray

1

1.66

2.33

3.28

  1. Cells were incubated under normoxic (N) or hypoxic (H) conditions with or without etoposide (e, 50 μM) for 16 hours. After the incubation, total RNA was extracted, submitted to reverse transcription and then to hybridization on the DNA microarrays or to amplification in the presence of SYBR Green and specific primers. a-tubulin was used as the house keeping gene for data normalization. Data are given in fold-induction.
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Molecular Cancer

ISSN: 1476-4598