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Table 4 Gene expression profiling in HepG2 cells incubated with or without etoposide under normoxic or hypoxic conditions after HIF-1α siRNA transfection.

From: Hypoxia induces protection against etoposide-induced apoptosis: molecular profiling of changes in gene expression and transcription factor activity

  N Ne He He + siRNA
CLK1 1 5.30 19.70 6.57
CGADD45A 1 1.33 0.71 1.01
IGFPB4 1 2.06 1.21 2.14
MCL1 1 1.69 1.16 2.09
BIRC2 1 0.73 0.41 0.44
BIRC4 1 0.76 0.35 0.25
BIRC3 1 0.93 2.52 0.35*
ING 1 1.08 0.41 0.43
JUN 1 1.50 1.23 2.66
MAP2K1 1 0.82 0.58 0.78
TANK 1 5.42 0.38 0.37
BAX 1 1.53 0.59 0.87
IGFBP2 1 1.13 0.77 0.92
MDM2 1 3.90 1.28 2.28
BAK1 1 2.35 0.69 1.69*
CDKN1A 1 2.61 0.65 1.41
GPX1 1 2.27 0.59 1.09
CDC2 1 0.89 0.29 0.35
PCNA 1 2.11 0.49 1.06
AKT2 1 4.46 2.31 1.90
E2F5 1 1.23 0.24 0.14
  1. Cells were transfected with 50 nM HIF-1α siRNA or non-targeting siRNA for 24 hours. They were then incubated under normoxic (N) or hypoxic (H) conditions with or without etoposide (e, 50 μM) for 16 hours before RNA extraction, reverse-transcription and cDNA hybridization, as described in Materials and Methods. Each value is the average of three ratio values calculated from three independent experiments. Mean ratios indicate a fold-increase or decrease in gene expression.
  2. Value higher than 1 + 1 SD, Value lower than 1 – 1 SD, * p < 0.05 vs He