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Table 4 Gene expression profiling in HepG2 cells incubated with or without etoposide under normoxic or hypoxic conditions after HIF-1α siRNA transfection.

From: Hypoxia induces protection against etoposide-induced apoptosis: molecular profiling of changes in gene expression and transcription factor activity

 

N

Ne

He

He + siRNA

CLK1

1

5.30

19.70

6.57

CGADD45A

1

1.33

0.71

1.01

IGFPB4

1

2.06

1.21

2.14

MCL1

1

1.69

1.16

2.09

BIRC2

1

0.73

0.41

0.44

BIRC4

1

0.76

0.35

0.25

BIRC3

1

0.93

2.52

0.35*

ING

1

1.08

0.41

0.43

JUN

1

1.50

1.23

2.66

MAP2K1

1

0.82

0.58

0.78

TANK

1

5.42

0.38

0.37

BAX

1

1.53

0.59

0.87

IGFBP2

1

1.13

0.77

0.92

MDM2

1

3.90

1.28

2.28

BAK1

1

2.35

0.69

1.69*

CDKN1A

1

2.61

0.65

1.41

GPX1

1

2.27

0.59

1.09

CDC2

1

0.89

0.29

0.35

PCNA

1

2.11

0.49

1.06

AKT2

1

4.46

2.31

1.90

E2F5

1

1.23

0.24

0.14

  1. Cells were transfected with 50 nM HIF-1α siRNA or non-targeting siRNA for 24 hours. They were then incubated under normoxic (N) or hypoxic (H) conditions with or without etoposide (e, 50 μM) for 16 hours before RNA extraction, reverse-transcription and cDNA hybridization, as described in Materials and Methods. Each value is the average of three ratio values calculated from three independent experiments. Mean ratios indicate a fold-increase or decrease in gene expression.
  2. Value higher than 1 + 1 SD, Value lower than 1 – 1 SD, * p < 0.05 vs He
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Molecular Cancer

ISSN: 1476-4598