Figure 2From: Molecular analysis reveals heterogeneity of mouse mammary tumors conditionally mutant for Brca1Characterization of Brca1 cell lines (A) Unsupervised Hierarchical Cluster of original tumors (0) and cell lines using the 1660 probes that in addition to passing intensity and missing-value filters had a minimum 2.5-fold expression change in either direction from the median value in at least 20% of samples (most variable genes). (B) Unsupervised Hierarchical Cluster of the original tumors 0_A1 and 0_B1 along with the cell lines derived from them. Cluster was based on the 914 genes reporting at least 4-fold difference in expression ratios between original tumor 0_A1 vs original tumor 0_B1. (C) Cell lines were stained with specific fluorescently-conjugated mAb to Cytokeratin 18, Platelet-derived growth factor receptor (Pdgfr) α or β, as indicated, in parallel with appropriate isotype control-matched mAb and analyzed by flow cytometry. In the histograms shown, the thick black line represents positive staining and the gray filled lines represent the isotype control-matched staining. The percent of positive cells after compensation is shown on the upper right of each panel.Back to article page