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Figure 5 | Molecular Cancer

Figure 5

From: Down-regulation of transforming growth factor-β type II receptor (TGF-βRII) protein and mRNA expression in cervical cancer

Figure 5

Quantification of TGF-β target genes c-myc and p15 gene expression using real-time PCR in cervical tissue. We evaluated c-myc and p15 mRNA in a total of 3 mice from each group. Samples from the following mice categories were employed: Nt-E, Nt+E, E7-E and E7+E mice. (A) Quantification of c-myc mRNA. The data are presented as the fold change in c-myc mRNA level normalized to the gapdh mRNA (endogenous control). Nt-E mice were used as calibrator. c-myc mRNA levels were very high in K14-E7 mice irrespective of estrogen treatment and were strongly increased in Nt+E mice as compared with the control Nt-E (P < 0.005) (Figure 5A). The difference in expression of c-myc mRNA between Nt-E vs Nt+E, E7-E and E7+E mice was statistically significant (P < 0.005). All significant differences are indicated by asterisks. (B) Quantification of p15 mRNA. The data are presented as the fold change in p15 mRNA level normalized to the gapdh mRNA (endogenous control). p15 mRNA levels were not increased in K14-E7 mice even in the presence of the hormone; however p15 mRNA levels were very high in Nt+E mice (P < 0.005) (Figure 5B). The difference in expression of p15 mRNA between Nt-E vs Nt+E, and Nt+E vs E7+E mice was statistically significant (P < 0.005). There was not statistical significance between E7-E vs E7+E P > 0.005. All significant differences are indicated by asterisks.

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