FOP-FGFR1 recruits and interacts with p85 PI3K at the centrosome in interphasic Ba/F3 cells. Costaining with anti-p85 PI3K (red, a,d,g) and anti-γtubulin (green, b,e,h) antibodies shows the recruitment of endogenous p85 at the centrosome, in FOP-FGFR1-expressing Ba/F3 cells, but not in wild-type (WT) or FOP-FGFR1 kinase-defective mutant (K259A) expressing Ba/F3 cells. Scale bar 5 μm. (B) FOP-FGFR1 interacts with endogenous p85. Immunoprecipitations with anti-FGFR1 antibody were done on lysates from Ba/F3 cells stably transfected or not with FOP-FGFR1, and were followed by western blotting with anti-p85 antibody to reveal the interaction. (C) FOP-FGFR1 provides a phosphorylated tyrosine in a YXXM motif at the centrosome. Costaining with anti-phospho-YXXM antibody (red, a,d,g) and anti-γtubulin antibody (green, b,e,h) shows phosphorylated tyrosine in a YXXM motif at the centrosome of Ba/F3-expressing FOP-FGFR1 but not in FOP-FGFR1 K259A or wild-type Ba/F3 cells. Scale bar 5 μm.