FOP-FGFR1 induces PLCγ1 recruitment at the centrosome during interphase and activates PLCγ1. (A) Immunofluorescence experiments with anti-PLCγ1 antibody (red, Aa,d), and anti-γtubulin (green, Ab,c) shows PLCγ1 at the centrosome and spindle microtubules of both FOP-FGFR1 or FOP-FGFR1 K259A- expressing cells during mitosis (arrows), and a specific recruitment at the centrosome of interphasic FOP-FGFR1-expressing cells (arrowhead). Scale bar, 5 μm. (B) Direct GFP fluorescence (green, Ba,e,i,m) of FOP-FGFR1 and its mutants, staining with anti-γtubulin antibody (magenta, Bc,g,k,o), anti-PLCγ1 antibody (red, Bb,f,j,n) shows recruitment of PLCγ1 at the centrosome by the different mutants. Scale bar, 5 μm. (C) Western-blot analysis with anti-pPLCγ1 antibody reveals activation of PLCγ1. Lysates from wild type Ba/F3 cells or Ba/F3 cells stably expressing FOP-FGFR1 or its mutants were used. Anti-PLCγ1 antibody was used for loading control.