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Figure 2 | Molecular Cancer

Figure 2

From: DNA-dependent protein kinase catalytic subunit modulates the stability of c-Myc oncoprotein

Figure 2

Effects of DNA-PKcs expression status on cell proliferation and radiosensitivity. (A) Survival curves of HepG2-H1 and HepG2-NC cells after γ-ray irradiation. (B) Growth curves of HepG2-H1 and HepG2-NC cells. (C) Growth curves of L02 cells measured after exposed to 0.1 pM TCDD for 2 weeks (L02-TCDD2W) or 4 weeks (L02-TCDD4W). (D) Focus colony formation assay. After treatment with 0, 0.01, or 0.1 pM of TCDD for two weeks, 1000 L02 cells were seeded into 60 mm diameter Petri dishes and cultured in normal growth medium without TCDD for 10 days, then fixed with methanol and stained with Giemsa solution. (E) Growth curves of L02/TCDD-H1 and L02/TCDD-NC cells, which were generated from L02 cells exposed to 0.1 pM TCDD for two weeks, then transfected with DNA-PKcs specific siRNA or nonspecific siRNA constructs, respectively. Cells grew in normal growth medium without TCDD after primary two weeks of TCDD exposure. (F) Cell proliferation activity as measured by MTT method. Student T – test: * P < 0.05; ** P < 0.01.

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