Figure 6From: Differential effects of energy stress on AMPK phosphorylation and apoptosis in experimental brain tumor and normal brainEffects of AMPK phosphorylation in astrocytes. a) Astrocytes were cultured with 3.0 mM glucose containing media in the presence or absence of 10 μM of compound C for 24 and 48 hours. Treatment with compound C inhibited AMPK phosphorylation and increased caspase-3 cleavage in astrocytes. b) Astrocytes were cultured with 1.0 mM glucose containing media in the presence or absence of 0.5 and 1.0 mM of AICAR for 72 hours. Treatment with AICAR significantly increased the number of astrocytes in a dose dependent manner as detected by fluorescence from calcein labeled astrocytes at p < 0.001.Back to article page