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Figure 1 | Molecular Cancer

Figure 1

From: Evidence for efficient phosphorylation of EGFR and rapid endocytosis of phosphorylated EGFR via the early/late endocytic pathway in a gefitinib-sensitive non-small cell lung cancer cell line

Figure 1

Intracellular distribution of pEGFR in the NSCLC cell lines. The gefitinib-sensitive NSCLC cell line, PC9 (A), or the gefitinib-resistant cell line, QG56 (B) was fixed and double-stained for LIMPII (red in b, h) or cathepsin D (red in e, k) and pEGFR (green) as described in the Materials section. Superimposed images of cathepsin D or LIMPII and pEGFR are shown in c, f, i, l. In both PC9 and QG56 cells, it is notable that pEGFR-positive small punctate vesicles are spreading in the cytoplasm and these vesicles are not colocalized with the LIMPII-, or cathepsin D-positive vesicular structures, and also pEGFR-positive punctate stainings are clearly seen in the nucleus. Bar, 10 μm.

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