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Figure 2 | Molecular Cancer

Figure 2

From: Evidence for efficient phosphorylation of EGFR and rapid endocytosis of phosphorylated EGFR via the early/late endocytic pathway in a gefitinib-sensitive non-small cell lung cancer cell line

Figure 2

Endocytosed Texas red-transferrin is downloaded into the SNX1-positive aggregated vesicular structure of early endosomes in the perinuclear region of gefitinib-resistant QG56 cells. The PC9 cells (A), or the QG56 cells (B) were fixed and double-stained for SNX1 (green in b, j) and LIMPII (red in c, k) as described in the Materials section. Superimposed images of SNX1 and LIMPII are shown in d, l. Each cell line was stained with DAPI (blue) to reveal nuclei. The merged confocal images as yellow color were quantified and presented as the percentage of total amounts of SNX1-positive vesicles per cell in C. The error bar denotes SD. In PC9 cells (A), SNX1-positive small vesicles are not colocalized with LIMPII-positive vesicles (d), however, in QG56 cells (B), part of LIMPII-positive vesicles colocalized with SNX1-positive early endosomes is seen in the perinuclear region (l). Furthermore, superimposed images of SNX1 and the internalized Texas red-transferrin in the PC9 cells and the QG56 cells are shown in h and p, respectively. The merged confocal images as yellow color as indicated by white arrowheads (h) or white arrows (p) were quantified and presented as the percentage of total amounts of SNX1-positive vesicles per cell in D. Note that SNX1-positive early endosomes form large aggregated vesicular structures in the perinuclear region (p) in QG56 cells, and that these aggregated structures are overlapped with Texas red-transferrin; however no aggregated vesicles are seen in PC9 cells (h).

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Molecular Cancer

ISSN: 1476-4598