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Figure 5 | Molecular Cancer

Figure 5

From: Evidence for efficient phosphorylation of EGFR and rapid endocytosis of phosphorylated EGFR via the early/late endocytic pathway in a gefitinib-sensitive non-small cell lung cancer cell line

Figure 5

Identification for an efficient pEGFR trafficking via early/late endocytic pathway in PC9 cells. The PC9 or QG56 cells were preincubated with EGF for 15 min on ice and then cell were chased at 37°C for 3, 6, or 15 min, and cells were fixed and double-stained for pEGFR (green) or LIMPII (red) as described in the Materials section. Superimposed images of pEGFR and LIMPII are shown. Each cell line was stained with DAPI (blue) to reveal nuclei. The white arrows indicate the colocalized LIMPII-positive late endosomes/lysosomes and pEGFR-positive cytoplasmic vesicular structures. The merged confocal images as yellow color as indicated by white arrows (c, d) at 15 min incubation were quantified and presented as the percentage of total amounts of LIMPII-positive vesicles per cell in B. It is notable that an efficient trafficking of pEGFR to late endosomes is seen in PC9 cells (a, b, c), but is not observed in QG56 cells (d).

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Molecular Cancer

ISSN: 1476-4598