Methylation analysis of the HLA-G promoter in the control and 5-aza-dC treated BG-1 cell line. (A) Schematic map of 450 bp of the HLA-G promoter region. Colored boxes represent enhancers and regulator binding sites: HRE = hypoxia response element; B2 = enhancer κB2; B1 = enhancer κB1; ISRE = interferon sequence responsive element; W/S= W/S box; X1 = conserved X1 regulatory box; X2 = X2 box; CAAT = CCAAT box; TATA = TATA box; ex1 = exon 1; Met-F and Met-R = forward (F) and reverse (R) primer binding sites. (B) Bisulfite genomic sequencing of 19 CpG dinucleotides of the region from -450 to ATG. Individual CpG dinulcotides are depicted as circles. Each row of circles represents an individual sequenced clone, either untreated (PBS) or treated with 50 μM 5-aza-dC (open circle = 100% unmethylated; filled circle = 100% methylated; %MET = percentage of methylation of each individual clone; %CONV = efficiency of sodium bisulfite treatment).