Formation and exocytosis of vacuole-like structures upon inhibition of EGFR activation in uPAR down-regulated cells: (A) Gefitinib led to reduced spreading and cell rounding which was more obvious in ACCS-AS cells. (B) There was also vacuolar formation observed as early as 10 minutes in ACCS-AS cell lines which continued and affected most of cell population with time. Representative vacuole formations on ACCS-AS cells (red arrowheads) are shown. Time-lapse videography also showed that formed vacuoles were exocytosed (see supplemented data). An example of masses speculated to be already exocytosed vacuole sacs are shown (green arrowheads).