Figure 4
From: The OGF-OGFr axis utilizes the p21 pathway to restrict progression of human pancreatic cancer
OGF has no effect on CKI p27 expression. BxPC-3 cells were synchronized by nocodazole (67 ng/ml) for 24 hours, and subsequently treated with 10-6 M OGF for 3, 6, 9, 12, 15, 18, or 21 hours. (A) Total protein lysates were resolved by SDS-PAGE and subjected to antibodies specific to p27. (B) Densitometic analysis revealed changes in p27 expression of less than 38% between OGF and sterile water treated values; none of these changes were statistically significant.